Toxicants and human sperm chromatin integrity

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NEW RESEARCH HORIZON Review Toxicants and human sperm chromatin integrity

The integrity of the paternal genome is essential as the spermatozoon can bring genetic damage into the oocyte at fertilization and contribute to the development of abnormal pregnancy outcome. During the past two decades, many assays have been developed to measure sperm DNA strand breaks, chromatin structure and compaction and assess the proteins associated with the DNA, as well as epigenetic m...

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P-1: Morphine Has Detrimental Effects on Sperm Parameters, Chromatin Quality and DNA Integrity in Mice

Background Morphine as a natural alkaloid (opiate) is considered as one of the problems associated with poor semen production and sperm quality. For the first time, this experimental study was carried out to evaluate the impact of intra-peritoneal injection of morphine on sperm parameters, chromatin quality and DNA integrity of spermatozoa aspirated from cauda epididymis of mice. MaterialsAndMe...

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Environmental toxicants cause sperm DNA fragmentation as detected by the Sperm Chromatin Structure Assay (SCSA).

Studies over the past two decades have clearly shown that reproductive toxicants cause sperm DNA fragmentation. This DNA fragmentation can usually be detected prior to observing alterations of metaphase chromosomes in embryos. Thus, Sperm Chromatin Structure Assay (SCSA)-detected DNA damage is viewed as the molecular precursor to later gross chromosome damage observed under the light microscope...

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effects of sperm chromatin integrity on fertilization rate and embryo quality following intracytoplasmic sperm injection

sperm chromatin integrity has been being recognized as an important factor in male fertility. during normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. however, using assisted reproductive techniques, particularly icsi, the natural selection is bypassed. therefore sperm with dna breakage have the opport...

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ژورنال

عنوان ژورنال: Molecular Human Reproduction

سال: 2009

ISSN: 1360-9947,1460-2407

DOI: 10.1093/molehr/gap087